How do you combine 3 or more compounds to increase the bioactivity e.g. synergy?
Dear Prof. Fatih,
Please find both individual and combined flavonoids against oxidized lipoproteins in vitro study. Hopefully this help
Best regards
Aly
Thesis Chapter 6-To investigate the antioxidant properties of dieta...
When you combine two and more compounds to assay a certain type of bioactivity, you need to consider three possibilities: synergy, additive and antagonization. For these reasons, I suggest that you search literature to define the chemical/structural relationship bewteen these compounds. For chemically similar compounds, you may consider additive; for structurally distict ones, you may have synergistic or antagonist, also possibly, additive. At the end, you need to optimize the concentrations.
Good luck!
Jianhui RONG
Testing for synergy is very difficult. You must combine compounds on an equal molar basis and then compare activity to equal amounts of the other 3 compounds to judge for increase of decrease in activity.
The increasing of synergy is depends to kind of used materials and those concentrations. Off course, you should consider the decrease of synergy that may be occurred, too. Thus, You must combine compounds on a specified molar and then test the biological activities.
Synergy is possible when the desired biological activity or pharmacological activity has several sites of action or targets.If the used compounds act on different targets , similar activity can b observed
- Badges
- Science topic