I'm making 2mg/ml collagen gels with 150,000 myofibroblasts. One hour after gel polymerization, I add 1 ml culture media and detach the gels from the well (24-well plate) with a pipette tip. I make sure the gel can move freely in media before I incubate it for 24h. Next day, when I check the gels, I find some of them attached back to the well surface! I'm using tissue culture-treated plates. Could this be why the gel sticks to the plate?
Any suggestions/advice would be appreciated!
As you suggested, I would suspect that the surface coating on the plates is causing your gels to re-attach. Have you tried using 'un-treated' or 'low attachment' plates?
I just tried using untreated dishes this week, the gel still attached to the dish... I read in a paper that they coated the dishes with 2% BSA before adding the gel, I'm trying that today. Thanks for your reply!
Are the gels the entire diameter of the dish? If so, they simply be too large. See what we did in the past . . . https://www.ncbi.nlm.nih.gov/pubmed/17537126
Yes, the gels are the entire diameter of the dish. I read that in some protocols the gel is transferred to a larger well but my first try of that didn't go so well. Interesting, I've not seen the use of Teflon rings for casting a gel before. I can definitely give that a try!
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