I have tried to break DNA by sonicator, but after 1% gel run, I saw 2 expressions?

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What are the journal lavalible for the research area in deep learning and domain breast cancer?

suggest any list of journals to publish my article with impact factor 3-4 impact factor

23 July 2024 6,219 2 View

How can the autonomy of learners be measured qualitatively?

Measuring learner autonomy is crucial for understanding and fostering independent learning skills. While quantitative methods provide valuable data, qualitative approaches offer deep insights into...

03 June 2024 997 0 View

How can the autonomy of learners be measured qualitatively?

How can the autonomy of learners be measured qualitatively? Measuring learner autonomy is crucial for understanding and fostering independent learning skills. While quantitative methods provide...

03 June 2024 2,743 0 View

How can the autonomy of learners be measured qualitatively?

Any theory or approach?

01 June 2024 2,805 0 View

Is there a way to estimate the covariance of a censored variable with a continuous variable in Mplus?

I am estimating the transactional associations of two LGC processes (maternal depressive symptoms and child externalizing behaviors) and would like to include the concurrent covariances of their...

29 May 2024 9,800 0 View

Instruments to assess gratitude in children and adolescents?

We are designing programs to promote gratitude in the school context and are looking for instruments to assess gratitude in school-age children and adolescents.

26 April 2024 3,143 0 View

Any suggestion to design primer to detect shRNA?

Hi there, I am trying to design primers to detect shRNA in the transfected cells with my vector. The structure of shRNA within the vector is stem-loop-stem, with a terminator downstream of it and...

04 April 2024 3,755 3 View

Hi! Please suggest the appropriate tests for hypothesis testing?

My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...

24 March 2024 1,932 4 View

Oxidation peak variations in Cyclic Voltammetry?

Why is one GCE showing three peaks while other giving two peaks in cyclic voltammogram under same conditions and parameters? Is it related to electrode cleaning?

12 March 2024 5,634 3 View

Qual é a avaliação Qualis dessa revista?

Procurei na plataforma Sucupira e não encontrei. Selecionei a área interdisciplinar de conhecimento. Obrigada.

04 February 2024 3,023 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,161 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How movement of energy in an ecosystem different from chemical cycling & movement of matter through an ecosystem differ from movement of energy?

How are the movement of energy in an ecosystem different from chemical cycling and movement of matter through an ecosystem differ from the movement of energy?

03 August 2024 6,134 0 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

How does matter cycling and energy moving through the ecosystem differ from one another and cycling of matter through the ecosystem?

03 August 2024 2,558 1 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Sabine Strehl

Can you please specify what you mean by "2 expressions" and explain your problem in more detail? Which size marker are you using and which fragment length size range would you like to obtain?

Sonia Shahataj Begum

I am using 100bp DNA ladder. I need sheared chromatin concentration between 100-1000bp.