There is a clot (likely looks like a platelet or fibrin clot) in one of the three bags after thawing of cryopreserved lymphocyte stored for Donor Lymphocyte infusions (Cryopreserved a month ago). The recovery of CD3 cells in the bag is within acceptable limits. There were no clots seen in any other previously thawed bags. The ACD ratio during the procedure and final ACD volume in the product was well maintained.
What could be the reason for this clot ?
Hi Soma Agrawal . It could be cryoprecipitate if that bag was not properly thawed.
How can i do a Root cause analysis of it? So that we prevent this from happening in future?
Mononuclear cells sometimes get activated in thaw cycle and start pro coagulant activity. This paper is helpful. Article Formation of fibrin clots in cryopreserved stem cell bags du...