Hi Osama, TA cloning leads to the insertion of a fresh amplified product into a so called TA cloning vector, but this cloning vector is not an expression vector because it does not contain a promoter upstream of the multiple cloning site. Therefore, you can go via TA cloning in case you want to insert an amplified product, perform a midi or maxi prep of that new TA vector with your insert, cut it afterwards out of the TA cloning vector by restriction digest and ligate it into your expression vector of interest and maxi prep this one. I hope that answer your question for what purposes TA cloning vectors are useful and why not for expression of your gene of interest. Good luck!

hi osama, I also want to produce Taq DNA polymerase, but I don't have thermus aquaticus strain. Do you know where can I find this strain or vector containing the polymerase gene. Thanks for your help.

Özlem

Hi ozlem

i purchased thermus aquaticus from DSMZ which Germany company. you can buy this bacteria online from this company

Thank you.