How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

Coupled hydric and mechanical equation COMSOL?

hello i am trying to study the mechanical deformation due to the humidity on a 3d volume formed of 2 phases. my equation are dw/dt =grad(Dm*div(w)) and the mecanical is sigma=E*beta*nabla(w)....

27 January 2021 5,201 1 View

Just face a Comsol issue dealing with magnetically hyperelastic material?

Hello all, I am just newly doing a simulation to test the deformation status of a magnetized rubber-like material, which is in-compressible, under a shifting magnetic field. Try to find the...

22 January 2021 7,427 2 View

Why is Abaqus showing me the deformed and undeformed shape at the same time?

17 January 2021 8,952 3 View

What can cause laser beam polarisation fluctuations?

Dear colleagues, We have observed large fluctuations in laser power in our setup and traced them down to fluctuations in laser beam polarisation. Attached is a schematic of the relevant part of...

14 January 2021 7,505 4 View

Data misrepresentation and Ignorance of socio-economic factors?

Do you think the majority of developing, underdeveloped, and even the developed countries lack in capturing the finer elements of behavioral data generated at various interactive and reactive...

13 January 2021 3,241 2 View