I want to ask what are the benefit of treating RNA sample with DNase I before cDNA and RT-PCR? how dose that affect result !
To get rid of DNA contamination from RNA samples. If DNA is contaminated, you cannot tell whether amplified DNA is derived from complimentary DNA or contaminated DNA.
what if you design primer that are located in two exon ?! what the benefit then ?!
Excess DNA may bind to primers used to generate c DNA
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How to calculate the RMSD values for a MD simulation using MOE?
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