Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is there a study of a long-term usage of muscimol and its impact upon human physical and mental health?

In my psychotherapeutic practice I have a client who decided to use really small doses of muscimol regularly. He gains it from dried fruit bodies of Amanita muscaria. Although he is quite enjoying...

14 December 2020 6,020 2 View

Covid 19 deaths- why the huge disparity between Asian countries which cannot be convincingly explained by public health measures?

Sri Lanka, Vietnam, Thailand, Cambodia and Laos for example are doing extremely well, but Indonesia, Philippines, Malaysia and India not so. It doesn't seem likely that this can be wholly due to...

27 October 2020 1,795 7 View

I see a lot of research right now that is inspired by COVID-19, even outside the medical field. Has your research focus changed due to the pandemic?

My question says it all. I am wondering if research agendas across many fields have changed due to the COVID-19 circumstances, and why.

06 July 2020 3,505 6 View

Can you help with an urgent survey about teaching during COVID-19?

Update: Data collection is closed and our manuscript has been published. Thank you to everyone who participated. You can find it at: Teacher Experiences in Converting Classes to Distance...

06 May 2020 1,701 46 View

Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

18 February 2020 2,386 1 View

Background noise in q-exactive orbitrap ms ?

Dear Colleges MS users. This is my very first thread on research gate. I have a new instrument - q-exactive orbitrap and i have experienced a high background ms noise in low m/z range. After...

29 January 2020 6,927 2 View

I'm looking for a laboratory-scale hydrogen separation membrane from the air?

dear all, I'm looking for a small-scale membrane for hydrogen separation from the air (or nitrogen). I need some information about available manufacturers. There is a lot of information about the...

15 December 2019 8,333 2 View

Is there anyone who could share the protocol for NLRP3 staining in the mouse paraffin embedded tissue?

Hello, I am looking for someone who performed already some IHC/IF staining in paraffin sections in mouse tissue for NLRP3. I used ab214185 and NBP2-12446 but no success so far. Thank you in advance

30 October 2019 4,354 1 View

Abaqus python, extract displacements of surface nodes, how?

Hi, I need to extract, from existing odb, displacements of nodes whhich belong to defined element surface. I use python. Till now I'm able to list the nodes labels and coordinates: N_set =...

27 March 2019 955 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

I have some problems with the mass transfer part of the CFD simulation of membrane distillation.Can anyone help me?

I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...

14 February 2021 379 2 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

What is the best bioinformatic tool to predict the interaction of peptide with bacterial cell wall membrane?

Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...

01 February 2021 1,351 3 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View