What do you recommend cheaper culture media instead of MRS media for Lactobacillus bacteria ssp. plantarum?
what are culture media on based milk or whey for the cultivation and enumeration of Lactobacillus spp. instead of MRS?
LAB such as Lactobacillus spp are known as fastidious microorganisms that cannot grow on simple mineral media supplemented only with a carbon source. In addition to carbohydrates (carbon source), culture media of LAB are usually supplemented with various
free amino acids, peptides, nucleic acid derivatives, fatty
acids esters, minerals, vitamins, and buffering agents. Thus few culture media are available for growing these bacteria such as MRS and M17. However, Yoo et al (2018) developed a cheap medium for growing a strain of Lactobacillus plantarum with a cultivation rate equals 96.2% of that of MRS.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960822/#__ffn_sectitle
MRS medium is rich in minerals and growth factors and hence is used for such fastidious lactobacilli.However, it could be replaced successfuly by a media based on whey enriched with minerals in many studies,
Main cost factor seems to be yeast extract. There are some cheaper yeast extract sources e.g. made from spent brewers yeast...maybe that is an option for you.
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