Hi everyone,

I am currently working with THP-1 cell line. I am trying to differentiate them and characterize cell population between monocyte versus macrophage. I did a basic light microscope image analysis but am looking for additional techniques. We do not have flow cytometry available. Has anyone done a fluorescent imaging to show positive differentiation with this cell line? Or are there any other suggestions?

Edit: I am looking for a more quantifiable analysis of showing monocyte to macrophage differentiation with PMA treatment aside from looking at phenotypic responses in imaging.

Thanks so much