Changes in tubulin expression in the same tissue?

J. Andrés Narváez-Pardo @J-Andres-Narvaez-Pardo

22 April 2023 1 3K Report

I have kidney and heart samples with the same concentration of protein, but when I run tubulin control, there are many differences between the samples. The membranes were stained with Coomassie blue, and no significant difference was observed between them. What could be wrong?

Can Kiessling

Just because the total protein expression was similar between samples and heart vs kidney doesn't mean the expression of a particular protein will be stable (across group / within group). You always should validate whether your loading control / reference protein expression is stable across your experimental groups.

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