Will there be any neuronal defect or increase in the death of Zebrafish embryo if the pH of E3 media is changed from 7.2 to 7.8 for dissolving the methotrexate.
Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...
09 February 2021 4,642 3 View
I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...
05 January 2021 9,882 3 View
Generally to differentiate between bacteria we use gram staining technique, broadly based on the composition of their cell wall. However archaebacteria have different cell wall composition, which...
14 December 2020 9,703 5 View
This case is important from a clinical point of view. Karyotype was normal. Advanced genetic tests were not conducted due to unavailability of such tests in the state as well as financial concerns.
07 October 2020 398 3 View
Suggest All, If any travel grand available for an independent researcher to present in conference i.e. American Society for Microbiology (ASM), USA
04 March 2020 5,629 2 View
Adding trizol directly into cell culture flask will damage the cells and what is the other alternative to add trizol into cells?
20 November 2019 5,316 2 View
In the area of Big data Analytics and Deep Learning.
18 December 2018 6,344 5 View
I am working on the project "Effect of dividend policy on firm's vale in India", my project is based on the study of one Nairobi student. Following are regression equation variables Value of...
09 November 2018 8,851 3 View
Hello, I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an...
18 July 2018 5,766 2 View
Is there any chances of coating of drug? or May at low pH my drug will release?
24 March 2018 4,827 3 View
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...
01 March 2021 736 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...
23 February 2021 6,756 1 View
I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...
23 February 2021 1,934 1 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...
21 February 2021 8,945 3 View
Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...
17 February 2021 3,368 2 View
Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...
16 February 2021 8,389 3 View