I have been growing cerebral organoids from an iPSC line and currently need a fixation and paraffin embedding protocol for them. I have looked into several papers but can´t seem to find a suitable one for paraffin.
Thanks in advance
I believe that it is more interesting to work with a cryostat.
Katherine.
Hi,
It is unlikely that you will come across research papers describing the fixation of cerebral organoids using paraffin. I attempted this approach in the past, but encountered difficulties. Firstly, cerebral organoids are already small tissue structures, and dehydration with alcohol further reduces their size. Secondly, when I attempted to embed the organoids in paraffin, they simply vanished. I could not observe the organoids in the resulting sections. Thirdly, even if successful in preserving the tissue, I would undoubtedly lose a significant number of slices.
Therefore, I recommend employing a different method for your work, specifically using a mixture of OCT (optimal cutting temperature compound) and 30% sucrose (in a 1:1 ratio) to freeze the organoids and subsequently slice them using a cryostat.
I hope this information proves useful to you.