what is the most efficient way and what do you think are the advantages or disadvantages of each of these isolation methods?
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View
I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...
25 March 2024 4,998 1 View
"Creative and Sustainable Initiatives to Combat Food Waste at the Consumption Stage" This Special Issue on combatting food waste through responsible consumption aims to gather the latest results...
29 February 2024 3,822 6 View
I have recently commenced research involving sulfate-reducing bacteria. For my experiments, I prepared an anoxic freshwater medium supplemented with trace elements, a vitamin mixture, and sodium...
29 January 2024 4,306 2 View
Hello, I would like to perform a western blot to detect a protein fused to GR (Glucocorticoid Receptor) and expressed from a 35S promoter in transgenic apple plants. I have never used anti-GR...
17 January 2024 7,806 0 View
Hi everybody. I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation. For...
16 January 2024 8,821 4 View
I`m trying to prepare a solution of water-cmc with the highest content of CMC possible for further processing, but I wanted to know how I can lower the viscosity so it will be easier to handle but...
04 January 2024 2,062 6 View
The liver sections look fragmented, how could the histological technique be improved to better observe this tissue under the microscope?
06 December 2023 7,388 3 View
During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and...
30 November 2023 6,702 1 View
Hi, I would like to mark the tip location of a NeurNexus probe. I know a good way to easily find the electrode location is to anesthetize the animal, perform an electrolytic lesion, wait a few...
21 October 2023 1,656 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View