I want to prepare a model solution containing glucose and cellulase enzyme of known concentration of glucose and enzyme protein. Some literature suggested loading of enzymes 10–30 mg/g, consistence of substrate 10–200 g/l , what does this mean?
Basically, if you use an enzyme by determining its specific activity [given in IU/ mg protein] in mg-amount, then you can say I have loaded 10, 20, 30...… 0r 100 mg cellulase with respect to a uniform amount of cellulose [say 100 g]. thus loading shall be 10 mg enzyme vs 100 g substrate].
For example if you have 10 g substrate in 1liter then you have to add 10X10 mg cellulase powder in this I liter solution. because 10 mg cellulase is required for 1 g substrates
The answer lies in your question only. It means 10-30 mg of enzyme can be loaded per gram of your substrate on protein basis wherein the substrate loading may range from 0.1 to 20percent. Now one can find the protein content in the enzyme by doing either Bradford or BCA assay and can find that in one gram enzyme how much protein content is there in terms of its BSA equivalents as BSA fraction V is used as standard usually in all protein assays.
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