I have performed a MTT viability assay to see how my cells (MCF-7 and HCT-116) respond to a drug. I had one control with cells + medium and another control with cells + medium + DMSO in different concentrations, the cells treated with the compound (wich is dissolved in DMSO) showed more viability than the cells treated with DMSO only in the same concentration. How is that possible? Is that normal to happen in a MTT assay?
Thanks!