29 October 2018 0 7K Report

Hi, I am looking for the best method for assaying cell viability in microfluidics channels. currently Calcein/PI staining is commonly used for measuring cell viability by microscopic counting of dead cells over live ones. Is there any other feasible method(s) available? Like, if I want to do a high-through-put, MTS or CCK8 can be used in 2D cultures. But if I want to specifically measure the viability of cells growing in 10 uM wide channels which method is highly preferred? Inputs are requested and are highly appreciated. Thanks!

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