HeLa cells were resistant to TGEV infection. However, when I incubate the Hela cells with about 1 MOI of TGEV, a lot of cells died two days after incubation. Why does this happen? is it due to the stress from this virus?
It is looking like viral cytotoxicity (usually apoptosis in response on presence of viral capsids or some else)
Hi Zaven. Thanks for your response. Do you know any ways that could reduce this kind of viral cytotoxicity to cells?
Hi
1. Try logarithmic dilutions of the virus in descending order until a dose that does not cause cytotoxicity is chosen.
2. You can change the cell culture.
3. Sometimes just a new passage of the same cell culture (thawed from liquid nitrogen) turned out to be more stable.
4.When working with picornaviruses, different strains of the polio virus at the same dose sometimes had different cytotoxicity.
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