T-test and Mann-Whitney U test on groups with different sample sizes and variances?

Hello I have 2 groups with different sample sizes of (experts: 5 to 8) and (Users: 28 to 35) answering 21 questions on a 7 points Likert scale about the importance of some dimensions for...

31 January 2021 3,885 7 View

What are the types of optimization problems that can't be tackled by meta-heuristic and heuristic algorithms?

Any decision-making problem when precisely formulated within the framework of mathematics is posed as an optimization problem. There are so many ways, in fact, I think infinitely many ways one can...

06 September 2020 9,415 14 View

Is it possible to reduce tensile strength and increase compressive strength by adding any admixture to concrete?

Hello, I want to know is it possible that the compressive strength of concrete increase by reducing tensile strength? if yes, how we can explanation and justification this issue?

30 April 2020 4,489 7 View

What are the plans for exams and assessments of universities during corona virus pandemy?

What alternative ways universities use for academic assesment of students?

29 April 2020 3,091 1 View

Is there a specific way to discretize a continuous model to any arbitrary multi DOF system and extract the mode shape matrix in abaqus?

27 April 2020 239 3 View

What is best sampling method for cross sectional studies?

I want to research on liver disease patients behaviours and habits in shiraz city. How can I calculate sample size accurately? Thanks

23 March 2020 9,974 5 View

Anyone in kolkata working of LNCaP prostate cells?

I am working with prostate cancer cells, i want to know who is presently working with PCa cell lines in viro and in vivo in kolkata.any leeds will of great help.

07 March 2020 7,831 1 View

How can I find the IDT point of the following TGA curve which is related to a clay soil?

The attached file shows the TGA curve of a clay soil. Can anyone help me how to recognize the initial decomposition temperature (IDT) from this curve?

06 February 2020 6,944 3 View

How to isolate dorsal root ganglia in rat at lumber region?

For pathological investigation of sensory and motor nerves in lumber region of rat how to isolate and preserve

04 November 2019 8,974 1 View

What's the procedure for validation test of a new design of product (Say Heat Exchanger)?

after 3D design of all components of the Heat Exchanger geometry we do assembly and finalization of the geometry, in CFD analysis we change the boundary conditions and it refers us to the...

27 October 2019 9,095 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View