I have some contamination problems in a mammalian cell line. There are some small microorganisms between cells in the everyday microscopy cell line check process. And any suggestions for us to eliminate the contamination.
Cultures contaminated with mycoplasmas elsewhere and then transferred into a laboratory are probably the leading source of mycoplasma infected cultures today. This is a common problem when using “gifted” cell cultures from other laboratories. (In the long run these “free” cultures may turn out to be the most costly cultures you will ever obtain.) The best way to avoid this problem is to purchase cultures only from reputable cell repositories that have vigorous testing programs and certify their cell lines as authenticate and free of microbial contamination. Cultures obtained from other sources should be kept quarantined in a separate incubator until mycoplasma test results are available. If a separate incubator is unavailable then the culture should be grown in a sealed flask and kept inside a plastic box with a cover or lid. Do not use unsealed dishes or plates. The suspect cultures should only be handled at the end of the workday after all other cell culture work is complete.
https://safety.fsu.edu/safety_manual/supporting_docs/Understanding%20and%20Managing%20Cell%20Culture%20Contamination.pdf
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