Cell culture L-DOPA analysis without HPLC?

More Erik A Miljan's questions See All

Did Einstein misunderstand the photo-electric effect?

Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...

19 July 2024 8,388 9 View

How can I download an article to my computer?

I have tried sharing, but it only provide a list of persons that does not include me. When I click on the download button, it does not seem to download it to my computer. Thank you

19 July 2024 1,814 3 View

Is light quantisation an illusion?

The photo-electric effect does not support quanta in light, since a light particle moving towards a surface cannot cause an electron to move away from the same surface at a right angle. Instead, a...

06 July 2024 2,288 35 View

Is gravity an EMERGENT phenomenon?

Newton found the law of gravity - but did not understand it. Fatio understood the law - but he was not accepted. Fatio assumed fast particles moving in all directions. Stability in planetary...

30 June 2024 9,284 28 View

I am seeking access to the CEB-FIP database or any similar repository containing comprehensive data on corroded prestressed steel?

Subject: Request for Access to CEB-FIP Database (or similar) for Developing ML Predictive Models on Corroded Prestressed Steel Dear ResearchGate Community, I am in the process of developing a...

02 April 2024 7,909 1 View

Is time dilation an old illusion caused by Michelson?

Michelson-Morley's tests were interpreted based on a particle model for light long time ago. This mistake caused an illusion of an ether wind effect in the arm transverse to light motion in these...

11 March 2024 2,499 7 View

Conditional logistic regression in R using clogit-function - log odds linearity?

I am doing a conditional logistic regression in RStudio using the clogit()-function from the survival-package. I want to assess linearity on the log-odds scale and I prefer not to use Box-Tidwell...

25 February 2024 9,671 3 View

Is time dilation a 137 years old illusion?

Time dilation has absurd predictions. So, it may be an illusion. Is it possible that this mistake has its roots far back in 1887, when Michelson did his famous tests? The interpretations of these...

30 December 2023 8,778 8 View

In need of Lambda max?

Can anyone assist in telling me the lambda max for the following compounds or a location to look them up? Brazilin Lawsone Vasicine Acetate Arjunic Acid Thanks

15 December 2023 7,736 0 View

Would an unprotected maleimide functionality survive TFA deprotection employed in standard solid phase peptide synthesis (SPPS)?

Please support with a reference if available, that would be much appreciated.

12 April 2023 9,900 2 View

Why does Tyrosinase suddenly become inactive when it was active previously?

Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...

06 February 2024 8,349 4 View

Can it be non-enzymatic digestion of BAEE in enzyme kinetics determination experiment?

Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...

24 December 2023 9,368 1 View

Someone have a precise protocol to perform DAF-FM Fluorescence in HUVEC cells ?

We are currently having some problems with DAF2 or DAF-FM fluorescence. We use a 5µM concentration and are not able to see any effect of a stimulation on DAF fluoresnce. When looking cells under...

16 November 2023 8,182 0 View

Does addition of L-arginine into DMEM increases the production of nitric oxide by RAW264.7 cells after LPS treatment?

If yes, what is the ideal concentration of L-Arginine in DMEM?

04 October 2023 1,856 0 View

How neutrophil isolation on ice affect the cells and culturing subsequently?

I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...

05 September 2023 6,293 0 View

Inquiry about secondary metabolite compounds production in specific bacterial strain?

Greetings everyone. During my master's research, I focused on exploring the potential of a specific bacterial strain to produce antibacterial compounds. To achieve this, I used the technique of...

24 August 2023 588 4 View

Can i re use same POLY L LYSINE after coverslip coating ?

it means we have a set protocol of using poly L lysine for coverslip coating, according to our protocol after 4 our of incubation in 37 C ,we are used to dicard the poly L lysine ,but as the poly...

09 July 2023 5,334 0 View

I had difficulty solving L-Lysine powder because some hydrophobic part was in there. What is the hydrophobic component, and how to solve them?

I have L-Lysine powder from Sigma Aldrich. Based on MSDS, the L-Lysine powder can easily be solved with water, but in my case, some hydrophobic parts can't be solved. It influences the UV-Vis...

12 June 2023 4,664 5 View

How made M9 culture media ?

Hi every body I made the ship's environment based on M9 culture media with the following ingredients( per 100 ml culture media ): KCL= 0.2 gr NaCl= 0.01 gr KH2PO4 =0.2 gr MgSO4= 0.001 gr Fe so4...

16 May 2023 8,115 0 View

What do two half lives mean when they are both reported for a single dtug?

In the article titled "FDOPA-(18F): a PET radiopharmaceutical recently registered for diagnostic use in countries of the European Union", two half-lives are reported for FDOPA....

19 November 2022 7,798 9 View