Hello
We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and should be mechanically stable at 37 ºC, which puts gelatin out of the list.
Any suggestions?
Thank you very much
- biological grade silicone
- https://biotium.com/product/covergrip-coverslip-sealant/ (check this out)
- Cytobond
- https://www.scigene.com/details.php?pid=1216
- https://www.fishersci.com/shop/products/cytobond-removable-coverslip-s/nc0662383
(no commercial interest)
Good luck!
@Sathya Srinivasan
You can try Eukitt® Quick-hardening mounting medium (Sigma-Aldrich cat# 03989). We frequently use it to glue glass coverslips inside cell culture plates (24 and 12 well plates), once it dries we pour cell culture media and seed cells on it. You can put a small droplet between glass parts to be glued let it dry before adding media. The base solvent is xylene based and after drying it is chemically quite neutral, so should not cause any issue if it comes in contact with cell culture media (our culture media comes in contact with it and our cells are growing fine).
Sathya Srinivasan thanks for those links, already looking into some of those products. Do you have any experience with Valap or rubber cement?
Amlan Barai can you please share how long the medium stays in contact with the Eukitt and what cells are you working with?
- Badges
- Science topic
- Similar topics
- Biological Science
- Histology
- Fixatives