Glucose simulation in GROMACS?

Hi all I am new to GROMACS and need help with some issues. (1) I am trying to run a simulation for glucose in water. The topology file for glucose was generated using AMBER and GLYCAM_06j-1...

04 May 2020 8,609 2 View

What is the refractive index of benzyl alcohol?

what is the refractive index of benzyl alcohol 99%? i will measure the sample through zeta sizer but the solvent did not exit in the library of the machine?

11 February 2019 1,779 3 View

Can i reused sample measured by UV spectroscopy ?

my sample is small, can i reused the sample that already measured and get the uv on it???? for other test used can i??

10 February 2019 941 4 View

What solvent of ethanolamine dissolved in?

i try to prepare 3 %wt of ethnaolamine ? i will take 3 ml of 99% pure 98% ethanolamine ? what solvent used to put 3 ml in it?

04 February 2019 6,813 3 View

If the plant extract sensitive to temperature and can be degrade ?

i try to synthesis nano particles using green synthesis but the temperature surround 100 C and i notify the color change which indicate the nanopaticles formation but i read in the literature...

04 February 2019 5,110 6 View

Why their is no change in color?

i try to synthesize platinum nano particles from hexacholoplatinumic acid with plant extract? it should change the color after few minutes from pale yellow to brown ? but with no change in...

07 January 2019 7,346 10 View

What is better concentration in green synthesis of metallic nanoparticles of plant extract?

i have plant extract and i dried it and get mass in gram after evaporation the solvent, now i want to synthesize metallic nano particles but how much mass in g, mg should dissolve in which solvent...

22 December 2018 9,636 3 View

What is the dilution factor?

salami researcher i dissolve 100 mg of plant extract in 5 ml of distilled water what is the dilution factor? thanks

17 November 2018 3,898 4 View

Do I need to monitor the pH of nanoparticles synthesis using green synthesis?

It's important to monitor the pH of nanoparticles synthesis using green synthesis? Or even know how much?

18 August 2018 2,563 5 View

How can I view my work " paper" and save my idea, while the publishing process takes a long time

If I have a new work and isn't submitted yet to any journal or conference. can I put it in arXiv and what is the advantage and disadvantage of this step?

01 January 1970 3,209 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View