05 August 2017 3 3K Report

Hello  

I tried to synthesize cDNA with norovirus.

After RNA extractiom, first of all, real time rt pcr was performed to calculate viral copy number. The high copy samples were processed with cDNA synthesis, and then the products were conducted conventional rt pcr to confirm cDNA.

The targeted band was confimed in PCR reaction with one step RP PCR kit (thermo)...but no band in respective reaction,  superscipt reverse transcriptasd and taq polymerase (recently purchase)

Does anyone help me?

More Ju Siyeon's questions See All
Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

Statistics: Controlling for pubertal status and gender?

Hi, I am not sure whats the best way to "control" for pubertal stage and gender in my analysis. Actually, puberty is classified in 5 stages (1=prepubertal to 5=fully grown). To make it easier and...

06 May 2020 9,363 3 View

How to make a 1500 watt high voltage(5-20KV) power supply?

I need to design and make a high voltage power supply to use in a lab made electrostatic precipitation with this specs: Input voltage: AC: 220 volt Output voltage: DC: 5-20 KV (Continuous...

09 April 2020 5,172 21 View

Experience with immunostaining with anti-Hepatocyte Specific Antigen (Hep Par-1) antibodies in frozen tissue?

Dear scientists, I would like to stain the formaldehyde-fixed, OCT-embedded human liver tissues with anti-Hep Par-1 antibodies (DAKO; clone OCH1E5) for IHC staining. Do you by any chance having...

20 February 2020 3,017 3 View

What causes wrinkles in thin films to disappear after heat treatment?

When I anneal the free standing thin film (>300 degree Celsius), wrinkles of the thin film disappear. What is the principle of this phenomenon? (This thin film is can be a single layer or a...

28 December 2019 8,410 4 View

What happens to the mechanical properties when annealing a metal thin film?

If the metal thin film is annealed, How do the mechanical properties such as tensile strength, hardness, Young's modulus change? The metal thin film is not alloy and thickness is less than 100...

21 August 2019 8,069 3 View

How do you calculate IC 50 for inhibition of tyrosinase activity?

How do you calculate IC 50 of different plant extracts in tyrosinase inhibition essay?

28 April 2019 8,241 2 View

Induce Point Mutation in Microbes (RpoB gene for Rifampicin resistance)?

Hi, I try to make Rifampicin resistance microbes (e.g., Corynebacterium) using point mutation in RpoB gene. The doubling time of Corynebacterium is ~3.5hrs, and spontanoeus mutation rate for...

01 April 2018 6,253 2 View

Possible reasons causing ribosomal RNA size shift?

Hi all, I ran my RNA gel (formaldehyde gel containing MOPS buffer) to check the integrity of ribosomal RNA isolated from primary mouse hepatocytes. The 18s and 28s bands are good, which may...

26 November 2017 5,882 1 View

I'm trying to fabricate some nanoparticles by n-butlLi, but I'm wondering how can I separate my Lithium helide salt from my nanopartcles in toluene?

see the reaction in the picture

10 July 2017 3,279 1 View

Similar questions and discussions
MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close