How to use the Embedded Region constrain on Abaqus CAE?

Hello fellow academics, For my Final Year Project, I am looking into different ways of modelling the interface between PVA and gelatin. I have successfully modeled the interface using the "tied"...

04 February 2021 8,698 1 View

Why does my Abaqus FEA simulation run forever?

Hello! I have recently been trying to model friction between two blocks of different materials in Abaqus FEA. I have started with a very simple model with two cubes of different materials next to...

17 October 2020 1,268 18 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

Any study on high spatial resolution water chemistry monitoring ?

We measured several water chemistry parameters (nitrate, DOC, EC, pH, Tre..) every 50m in the stream network of six headwater catchments. Has anybody done that before, and what geostatistical...

23 March 2020 7,355 12 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

When you have a nanoparticle stabilized by a polymer, is it more stable than a nanoparticle stabilized by a non-polymer ?

Any explanation or reference ? Thanks !

07 August 2018 4,705 6 View

Question about Crispr Cas system construction?

I want to constructed Crispr Cas system in archeae and I need to know which promoter and terminater I should use to express RNAguide. Indeed, if I understand well, promoter should be one for tRNA...

17 October 2016 1,260 3 View

How can I use a node displacement in a FRIC subroutine (in Abaqus) ?

Hello everyone, I am trying to model a friction behaviour in Abaqus. To do that, I use the FRIC subroutine. The model works fine in loading, but it changes when unloading, so I have to use a...

25 October 2015 3,984 3 View

Does anybody have some experience in the FRIC subroutine in Abaqus?

03 July 2015 8,136 9 View

How can I choose the constraint enforcement method to model receding contacts in Abaqus?

09 June 2015 5,837 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Are there SDMs that can take in percentage data?

Hi everyone, I am wondering if anyone knows of a species distribution model that can take in percentage data (i.e. presence data with prevalence values attached to them)? The dataset I have...

18 February 2021 7,312 3 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View