CD68 antibody - FAQS.TIPS

More Luís Lima's questions See All

Multiple labeling in IHC with two mouse monoclonal Abs?

Which is the best way to performe multiple labeling (dual staining) IHC with two mouse monoclonal Abs, to detect two molecules in the same cell compartment? Is this possible or is better to try IF?

01 February 2012 3,664 9 View

Protein extraction protocol from FFPE

I need a good protocol to extract protein from formalin-fixed paraffin embedded tissues for Western Blot.

11 December 2011 4,043 1 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

To what degree does the immunohistochemical (IHC) evaluation of Ki-67 hold diagnostic value beyond neoplastic pathologies?

In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...

25 June 2024 7,825 2 View

Help with mounting meningeal tissues with Prolong Diamond?

Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...

05 June 2024 624 2 View

Hello! Does someone know of any antibody for neurotensin?

I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.

22 May 2024 3,565 7 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Shandon Sequenza leaves my IHC samples with spots that have not been stained?

I have been using Shandon Sequenza for the last two years with no problems. Lately i have noticed small spots under a microscope that have not been stained by DAB (only hematoxylin as a contrast...

28 April 2024 2,530 1 View

Vivek Anand Manivel

DAKO it is.. look for the number of publications it will be more obvious.

mert çalım

I suggest Novocastra. But due to Dako and Novocastra have common antibodies a lot, probably it won't be any difference. We have good results with Novocastra.

Veronica Molina Hernandez

What do you think about human MCA874 (Serotec)?. We use it in bovine tissues.

Luís Lima

I tried both and Dako works better. Thanks.

Ayman EL-Meghawry EL-Kenawy

Dako works better