Agarose EMSA for aptamer binding: no bound complex band?

Hey there, Due to limited resource, we are trying to assess the interaction of a 50mer aptamer with a 25kDa protein domain. The protocol we are using is unusual in that the gel is 2.5% agarose...

23 July 2020 8,192 8 View

Design of PCR primers for extremely short oligonucleotides?

Hello there, hoping you are well, I have reached a point in my current project where I need to amplify very very short (50mer) non-coding ssDNAs. I understand the options I have available...

20 July 2020 1,558 5 View

Can the overall similarity of a standard mass spectrum to that from an unknown be used for identification without using the fragmentation ion ratio?

Here is situation: Compound is run on GCMS give a mass spectrum. Old standards are used from a library. The identification is made by observing with eye that the two OVERALL patterns are...

11 February 2020 3,965 3 View

What operations are necessary for a proper identification of a compound using optical microscopic methods?

It is my understanding that the forensic identification of a controlled substance using optical microscopy requires more information that just the classification of the type of crystal formed....

27 January 2020 5,751 2 View

What software is available to model the impact of design change in complex products?

This question relates to research into the management of engineering design information and the impact of innovation. Complex products such as aircraft, trains and ships are high-value, long-life...

18 December 2019 316 3 View

What software is available to model the impact of design change in complex products?

Complex products such as aircraft, trains and ships are high-value, long-life assets that are typically managed as a fleet over a period of 20 to 30 years. Managing technology upgrades...

18 December 2019 4,710 3 View

cannot delete question?

11

27 April 2018 5,230 4 View

In SPSS, how can I adjust data to remove the effect of a covariate?

In brief overview, I have grown some plants and measured plant height (amongst many other traits), but my plants have been damaged by frost. I gave each plant a frost score between 0-5 as a...

05 March 2018 5,520 4 View

Haemoxygenase-1: does anyone have expertise for what solvent to use to store this protein in at -80 degrees?

Hi We recently started doing some ELISAs to look for levels of this protein, but are finding that reconstitution in water (as instructed by manufacturer) probably didn't completely solubilise the...

09 May 2017 7,815 1 View

What computational advantages does computing a feasible solution have compared to computing an optimal solution?

Under the same conditions of previous question

11 December 2015 5,915 2 View

Does anyone know where I can purchase human lacrimal gland secretory cells?

Hi, I'm looking to find a human lacrimal gland cell line, consisting of secretory (serous/acinar) cells, as I wish to design an in vitro model of the lacrimal gland. I have checked ATCC and all...

09 March 2020 7,983 6 View

What is the best protocol for albumin detection by flow cytometry?

I'm differentiating hiPSCs into hepatocyte-like cells and I want to detect albumin by flow cytometry. I'm wondering what is the best protocol to do it. I read that for detection of secreted...

06 July 2019 1,065 3 View

Is it possible to create lenses , which can be absorbed in eye?

Many people use lenses in order to experience different eye color & also instead of glasses and etc. It is important to bring lenses out of the eye ,daily otherwise side effects happen like...

09 October 2018 3,631 4 View

What is the working mechanism of NBD C6 Ceramide?

I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?

08 September 2016 6,101 1 View

Solubility of Brij 52 in water and ethanol??

I am working with Brij 52 and I would like to know the solubility in water and ethanol. Can anyone help me?

21 April 2016 9,448 2 View

What are the possible techniques for determining drug release of thin ocular films without using the dissolution apparatus?

For the determination of drug release of ocular films (0.5 cm x 0.5 cm), what are the possible methods that can be used? Using dissolution apparatus would be too big for such a small sized film,...

20 April 2016 6,974 3 View

Solubility of Brij 52 in water and ethanol??

I am working with Brij 52 and I would like to know the solubility in water and ethanol. Can anyone help me?

03 April 2016 9,033 2 View

What is the best solvent to dissolve TiO2 white powder???

what is the best solvent to dissolve TiO2 white powder???

03 April 2016 9,654 3 View

What are the possible techniques for determining drug release of thin ocular films without using the dissolution apparatus?

For the determination of drug release of ocular films (0.5 cm x 0.5 cm), what are the possible methods that can be used? Using dissolution apparatus would be too big for such a small sized film,...

03 April 2016 5,412 4 View

Hi Dear all, I would like to ask how can find the best solvent for mix gas (acetic acid, formic acid,CH4,..) ?

20 February 2016 9,394 2 View