I would like to claim authorship of the article below but have been unable to do so?

Experiences of sleep hygiene education as an intervention for sleep problems in children with developmental disabilities : findings from an exploratory study

07 December 2020 3,607 3 View

Atlas-TI 8: How come some codes with no quotations and no density cannot be deleted?

Hi, I am cleaning and reorganizing some data and there are some codes that I removed all quotations from and still, I cannot remove them. The option to delete them is not available. Does anybody...

28 September 2020 409 2 View

How to submit annotated genomes to NCBI?

Hello, We would like to submit annotated bacteriophage genomes to NCBI but we have a bit of a hard time to fully understand the process. We already created the bioproject and biosamples and...

07 September 2020 715 3 View

If using polymers for flocculant in a waste water treatment plant how often should they have a tox test done?

A wastewater treatment plant is adding dual polymer do reduce TSS, as per their permit they must do tox tests for this. How often and which tests should be done? Thanks

18 March 2020 4,416 3 View

Mir-prefer failed on bam sorting ?

Hello, I am trying to run the pipeline MiR-PREFeR I have run the command pipeline and I have this error after the "Done sam2bam" I have this error : Error occurred when filtering regions and...

04 March 2020 5,930 3 View

Why does my qPCR look like this?

qPCR assays that previously worked perfectly are not working any more and the results are, in a word, baffling (see picture). The amplification appears to work fine until cycle 30 or 35 and then...

20 November 2019 6,650 1 View

Who should be the first author?

After working tirelessly with your graduate student to carry out a research and making serous input in a journal article involving both of you., who should be the first author of this publication?

13 June 2019 4,741 5 View

Can someone suggest a tool for theming qualitative data from a systematic lit review?

I am exploring the composition of an holistic palliative assessment for my dissertation

29 April 2019 4,599 6 View

Searching DNA sequence of artificial intron (ie, splice site study) for inserting in plasmid (for drosophila cell system)?

I want to construct a plasmid (for Drosophila cell system) containing a intron in order to study splicing process. In detail, I would like to insert wether a weak or a strong splice donor site...

27 March 2019 9,210 1 View

Can anyone explain why Jisc now uses the term 'digital capabilities' rather than 'digital literacies'?

I am researching student understandings of what it means to be digitally literate and trying to see if they can move beyond being able to use different technologies and include other elements. The...

21 March 2019 6,092 4 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,406 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 3,835 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,133 1 View

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08 January 2019 3,512 5 View

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31 December 2018 8,083 5 View