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Questions related from Julie Decock
Hi, we are trying to transfect breast cancer cell lines with several cell penetrating peptide-siRNA complexes but it seems that our peptides are not taken up by the cells. We use several CPPs:...
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I am trying to culture the HMEpC cell line but the cells are slowly dying. Upon arrival of the cell line from the supplier, I put them in a T75 flask using the Human Mammary Epithelial Cell Growth...
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I'm looking into various quantification methods for blood vessels in FFPE tumour tissue. We have endomucin stained slides with Alexa labelled 2airy Ab. I have noticed some people count the number...
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I'm looking into how you could do siRNA transfection of cells once they are cultured as spheroids in a 3D matrix (collagen). Has anyone tried this before? Would you use the transfection reagent...
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Has anyone got a good protocol for casein zymography for detection of MMP7 activity and which has been successfull? Or any specific tips/tricks? I use cell line conditioned media and have been...
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I'm looking for a protocol for TCR cloning into a retroviral vector with peptide-stimulated T cells as starting point. I was told by a colleague to first clone the TCR alpha and beta chain using...
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