How to measure acid values of unsaturated polyesters?

I've been using the standart AATM 109-01 (2006) procedure to determine the acid values of unsaturated polyesters. In this procedure solutions of Phenolphthalein and Potassium Hydroxide in etanol...

11 November 2020 7,768 6 View

Does anyone have a reliable protocol to assess cell viability in commercially available GelMA (Gelatin methacryloyl) ?

Hello all, I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing...

13 September 2020 9,086 1 View

Which are the best loading controls for Western Blot excluding the cytoskeleton ones?

I need to use a loading control for Western Blot that neither includes a cytoskeleton protein or a protein that interacts directly with the cytoskeleton. My issue is that I incubate cells with...

27 August 2020 2,735 11 View

Does anyone know or can resolve this kind of Internal Error on Anylogic?

I am currently working on a Urban Traffic Simulation on Anylogic and sometimes when the simulation is running, this error appears and everything stop: "Exception during discrete event...

23 May 2020 8,461 0 View

What is the header of the Gaussian input file for calculating two distinct molecules?

I'm doing simulations with two different molecules, but it always ends up falling into the same problem, it seems that the Gaussian doesn't balance the molecules when I calculate the cation and...

03 March 2020 7,750 2 View

No title

03 March 2020 4,189 1 View

No title

Hello, i need to generate a few parameters of excitation states in a series of molecules. I am using g09. I have a opensuse (linux) with 24 cores, Dell setup (i believe it configures a...

14 February 2020 9,488 5 View

Is anyone interested in setting up a distance research group on agricultural risk management?

I am looking for researchers who are interested in the theme "agricultural risk management" to do a research group at a distance. Each addresses the experiences of local risk management, from...

08 December 2019 8,879 29 View

Are there any articles about impulsive buying and grandparents?

Hello! I'm taking an internship in Social Psychology Laboratory and researching Impulsive Buying. However, it seems that little research gathers information about these two variables together. Me...

07 October 2019 4,982 5 View

How high is the prevalence of point S and / or posterior epistaxis?

Recently, we have seen and read reports on posterior epistaxis origin being frequently located on the upper nasal septum (1). On my personal experience, this is by far the most frequent source on...

27 September 2019 7,231 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View