Can anti-6xHis antibody detect 8xHisTag?

Hi researchers, As the title shows, recently I made a construct with a 8xHis tag at N ternimal of HBx (154 aa). The voctor is pCDNA3.1+ and the sequencing result showed everything was correct. I...

10 November 2017 6,357 5 View

Anyone use this PCR mycoplasma detection protocol and get a 100bp product?

Hi researchers, I'm using a PCR mycoplasma detection protocol which is found online and it works well for me. Link as...

09 October 2017 4,263 4 View

What is the difference between I-TASSER and QUARK protein structure prediction?

07 August 2017 2,179 2 View

Positive control gene for real time PCR in IL6 stimulated cells?

Hi there, I want to treat several liver cell lines with IL-6 and then check some genes' expression at mRNA level using real time PCR. Is there any recommendation for positive control in this...

06 July 2017 5,939 3 View

Why cannot detect protein after over-expressing by lenti-virus?

Hi researchers, I want to over express a protein in Hep3B cell line. The expressing vector I use is attached here. The packaging vectors are pMD2.G, pRSV-Rev and pMDLg/pRRE. The amount I use for...

06 July 2017 1,628 4 View

Is Swiss prot ID unique for each transcript?

03 April 2017 2,260 3 View

Secretory protein with Flag at N-terminal?

Hi researchers, I made a construct with a Flag tagged to the N-terminal of my aim protein. I've already done the lentivirus infection and selected for over 2 weeks. I did a real time PCR to check...

31 December 2016 6,854 7 View

What is the size for BMP2 protein in western blot?

31 December 2016 7,301 4 View

Which part of the siRNA matters most?

Hi all, I want to order one siRNA from one publication. In the publication, they used 3 siRNAs and I want to order the most efficient one first. This is a 19nt siRNA. But when I do the blast,...

09 October 2016 381 4 View

What does XM_xxxxxxxxxx mean in NCBI?

Hi researchers, When I do primer blast, the result will show me an ID of the gene that my primer will bind to. If I'm not wrong, NM_xxxxxxxxxx means it's a human sequence. Then what does...

08 September 2016 4,079 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View