Can anti-6xHis antibody detect 8xHisTag?

Hi researchers, As the title shows, recently I made a construct with a 8xHis tag at N ternimal of HBx (154 aa). The voctor is pCDNA3.1+ and the sequencing result showed everything was correct. I...

10 November 2017 6,357 5 View

Anyone use this PCR mycoplasma detection protocol and get a 100bp product?

Hi researchers, I'm using a PCR mycoplasma detection protocol which is found online and it works well for me. Link as...

09 October 2017 4,263 4 View

What is the difference between I-TASSER and QUARK protein structure prediction?

07 August 2017 2,179 2 View

Positive control gene for real time PCR in IL6 stimulated cells?

Hi there, I want to treat several liver cell lines with IL-6 and then check some genes' expression at mRNA level using real time PCR. Is there any recommendation for positive control in this...

06 July 2017 5,939 3 View

Why cannot detect protein after over-expressing by lenti-virus?

Hi researchers, I want to over express a protein in Hep3B cell line. The expressing vector I use is attached here. The packaging vectors are pMD2.G, pRSV-Rev and pMDLg/pRRE. The amount I use for...

06 July 2017 1,628 4 View

Secretory protein with Flag at N-terminal?

Hi researchers, I made a construct with a Flag tagged to the N-terminal of my aim protein. I've already done the lentivirus infection and selected for over 2 weeks. I did a real time PCR to check...

31 December 2016 6,854 7 View

What is the size for BMP2 protein in western blot?

31 December 2016 7,301 4 View

Which part of the siRNA matters most?

Hi all, I want to order one siRNA from one publication. In the publication, they used 3 siRNAs and I want to order the most efficient one first. This is a 19nt siRNA. But when I do the blast,...

09 October 2016 381 4 View

What does XM_xxxxxxxxxx mean in NCBI?

Hi researchers, When I do primer blast, the result will show me an ID of the gene that my primer will bind to. If I'm not wrong, NM_xxxxxxxxxx means it's a human sequence. Then what does...

08 September 2016 4,079 2 View

For secreted protein, can we do western blot using cell lysiate instead of ELISA of cell culture supernatant?

I want check the protein level of BMP2 (Bone morphogenetic protein 2). I see someone use cell lysiate to do western blot while others use cell culture supernantant to do ELISA for BMP2. I know...

08 September 2016 1,510 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Anyone know how to contact Raymond Streater?

He is emeritus in the mathematics department of King's College London. The email address listed on their web site is not functional. The department has no way of physically contacting him due to...

15 February 2021 5,529 1 View

How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View