Canine embryonic stem cells?

More Christiane Kafarnik's questions See All

Has anyone experience with isolating keratinocytes from volar (planatar) skin from either human or porcine tissue?

Our project involves the characterization of volar skin - the skin of the foot (heel). I have been trying to isolate keratinocytes from volar skin from pig. But to date not very successful / very...

12 May 2021 2,826 0 View

Can anyone recommend a pedigree software?

We would like to start using a program to build pedigrees for our genetic CJD and dementia cohorts. The program should allow to incorporate and search for clinical data.

05 May 2021 8,154 2 View

Which leafs of a corn plant to choose for SPAD measures over time ?

Hello everyone, I want to measure the chlorophyll content of a corn plant during its growth (3 months). Wich leaves of the plant do you choose for those kinds of measurements ? Do you change it...

19 April 2020 8,604 1 View

Why does fluorescent WB detection fail while chemiluminescence is fine?

I have a simple western blot of CD31 in HUVEC lysate. With an anti-mouse-HRP secondary Ab I get a good signal/band. With fluorescent secondary antibody there is no signal. I have tried multiple...

05 August 2019 6,219 3 View

HEllo, someone for helping me with carbohydrates content calculations with the anthrone method ?

I have 1 ml of chlorella vulgaris pellet + 2 ml of ethanol 70% , after incubation , i took 50 µl of the supernatant + 950 µl of water and 2 ml of anthrone. Now i have a standard curve of...

04 July 2018 3,420 2 View

I need to extract total RNA from protoplasts, could anyone help me with a detailed protocol?

Working with protoplasts isolated from Arabidopis and transfections are done at a concentration of 10⁴ cells (which is the total cells that I have in approximately 50 microliters of pellet).

22 June 2017 9,695 1 View

In the ChIP-qPCR assays what is the best method for DNA quantification and how should I normalize my data?

I started using ChIP in my analysis a short time ago and I have a lot of questions about how to normalize my data, what percentage of Input should I use and what is the best method to quantify my...

14 June 2017 7,373 3 View

Can anyone provide advice for the purification of His-tagged protein from ExpiCHO supernatant?

From transient e get very high levels of secreted recombinant protein with a N-terminal his-tag, but our purification results are poor (less than 5% yield). We have tried desalting the...

29 January 2017 9,756 12 View

Neuroimaging meta-analysis with GingerALE: how to interpret labels in the output file?

Hello, I already asked this question in the Brainmap forum which belongs to the GingerALE software. However, so far I haven't got any reply over there and I'm in need of an answer. I hope you can...

10 April 2016 4,730 3 View

Are there studies about Undergrad music performance students that modify their career path because of extreme Music Performance Anxiety ?

MPA & Career choice

23 September 2015 8,525 9 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Leo Cornelius

Commcercialy? No.

To a lab with the proper qualifcations? Yes.

A veternary proffesional may be able to sorce them however it varys from country, l to countryook up you areas local guidlines on this.

Christiane Kafarnik

Ashraful Kabir

So far I know, this is not available. Selective breeding is sufficient in this case.