I need to extract total RNA from protoplasts, could anyone help me with a detailed protocol?

Christiane Eliza Motta Duarte @Christiane-Eliza-Duarte

23 June 2017 1 10K Report

Working with protoplasts isolated from Arabidopis and transfections are done at a concentration of 10⁴ cells (which is the total cells that I have in approximately 50 microliters of pellet).

Reza Hadavi

I hope this helps you.

http://www.dbs.nus.edu.sg/lab/wong/Protocols.htm

https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-1-93

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142859/

good luck

Badges
Science topic

More Christiane Eliza Motta Duarte's questions See All

How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

In which dry organic solvent/solvent mixture molten 1H-imidazole could be precipitated at 100%?

I am trying to isolate an allkylated imidazole from a mixture containing unreacted 1H-imidazole. The problem is that imidazole is slightly soluble in my targed molecule. Furthermore, I need to...

04 November 2020 4,513 3 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

What can be causing uneven focus on IHC-P stained slides?

Hello, I have been performing some IHC staining on parafin embeded tissue. All was good until recently. In the latest slides I have prepared, when looking under the microscope, some areas need to...

12 February 2020 9,906 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

Why does fluorescent WB detection fail while chemiluminescence is fine?

I have a simple western blot of CD31 in HUVEC lysate. With an anti-mouse-HRP secondary Ab I get a good signal/band. With fluorescent secondary antibody there is no signal. I have tried multiple...

05 August 2019 6,085 3 View

[COMSOL 5.1] Simple FSI simulation not converging. Can someone please take a look at my model?

Hello, I'm a newbie on CFD and starting to learn how to use COMSOL. I tried to modify one example from COMSOL but it isn't working. I think it has something to do with Inlet velocity, but i...

11 April 2019 2,662 3 View

How can I use FSI+Piezoelectric module in COMSOL Multiphysics?

I am trying to model Piezoelectric effect using vortex induced vibrations on a cylinder attached to a fixed beam while the end of the beam has a piezoelectric patch in 2D, but when I add the...

27 March 2019 6,694 3 View

Why the isotopes tends to concentrate in tumours?

What happens in the cancer cells or diseases to ''attract isotopes''

12 November 2018 7,361 3 View

How to intrepert and report two-way anova + post-hoc analysis data?

I have an animal experiment with 4 different groups: A) control diet + vehicle B) control diet + drug C) experimental diet + vehicle D) experimental diet + drug. We then measure the expression of...

25 June 2018 3,828 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View