23 June 2017 1 10K Report

Working with protoplasts isolated from Arabidopis and transfections are done at a concentration of 10⁴ cells (which is the total cells that I have in approximately 50 microliters of pellet).

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Help: Troubleshooting Metash UV-Vis (MetaSpec Pro), Negative Absorbances <230 nm?

Tl;dr - As of last week, the UV-Vis instrument at my job only records negative absorbance below 230 nm for any sample when zeroing methanol to correct for solvent. Attached spectra of the same...

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Do you believe in this theory? If so, what are your thoughts about Putin being the new Stalin?

Behind his senseless justification of the Ukraine invasion ,many believe that Putin is hiding his truly intentions , which is to reconstruct the URSS.

01 October 2023 3,701 0 View

QPCR related problem?

I have a situation which quite do not understand. We are trying to get a qPCR to work. We extracted RNA and synthersized cDNA from have 5 Wts samples, 3 iRNA samples, and 1 genomic WT control. We...

24 August 2023 2,050 2 View

Can anyone share a protocol related with the Purification Protein expressed in Nicotiana benthamiana leaves agroinfiltrated with Agrobacterium ?

Hello, I am working on the extraction from Total Protein Soluble and then purification of protein expressed in Nicotiana benthamiana leaves agroinfiltrated with Agrobacterium. Could you suggest me...

08 June 2023 7,540 0 View

Can you change the PDF of my work in your system, please?

Dear friends, scholar google warn me (https://scholar.google.pt/citations?view_op=view_citation&hl=pt-BR&user=g3V8bNsAAAAJ&citation_for_view=g3V8bNsAAAAJ:5nxA0vEk-isC) that an...

05 May 2023 5,250 2 View

I'm writing a scientific report for the first time for uni. Which statistical test should I do and how?

The data consists of a control and the measurements taken at 2 weeks, then another control and measurements taken at 4 weeks. The data is for 2 groups so there's 2 sets of data for the different...

02 April 2023 3,385 2 View

Is the multiple correlation coefficient (R) undefined in the case of negative determination coefficients (Artificial Neural Networks)?

I noticed that in some very bad models of neural networks, the value of R² (coefficient of determination) can be negative. That is, the model is so bad that the mean of the data is better than the...

19 March 2023 3,327 4 View

Does anyone knows how to solve system of ordinary differential equation using Aspen Custom Modeler discretization?

Please show me an example of code

10 October 2022 9,831 3 View

How to etch/pattern alumina?

I have alumina deposited on SiO2 substrate. How to pattern the alumina layer? I was thinking RIE with SF6 and CHF3, but this will etch into my substrate. Is there another method besides liftoff?...

08 September 2022 2,883 2 View

How to interpret the Vickers hardness data?

I have done Vicker's hardness analysis with titanium and thin-film coated titanium and I'm trying to show the hardness of the coated titanium. I plotted the graph of hardness versus load from 0.1...

09 February 2022 1,146 10 View

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