02 February 2017 1 6K Report

I am preparing presentation for my upcoming conference in India. Anyone help me to provide information about difficulties, challenges in using miRNA probe for cancer diagnosis. Thanks in advance

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Anyone familiar with variant formation during the change of LB broth volume (5 ml to 100 ml) for experimental evolution study?

I am doing sub-culture of my bacteria (WT) every 16 h in 5 ml LB broth with antibiotic for 30 days. For whole genome sequencing, I should change the LB broth sub-culture 5 ml to 100 ml to collect...

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Embryogenesis

What is the evidence for the generally taught "primordial germinal layer" hypothesis? Is it sufficient to sustain the hypothesis/dogma? Are there any indications that contradict the...

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Embryology: Do the 3 lineages give rise to layers in colon with 3 distinct patterns of ancestral somatic mutations?

Is this really what happens? If so, the three lineages would give rise to layers in organs such as the colon with three distinct patterns of ancestral somatic mutations. Alternately, organs could...

07 August 2014 283 3 View

How to identify the function of hypothetical proteins?

I have isolated and identified more than 40 hypothetical proteins from E. coli by using MALDI-TOF and LC-MS/MS. Hypothetical proteins are cloned, over expressed and two proteins are characterized...

03 April 2013 3,036 5 View

No protein expression after IPTG induction.

I have cloned my gene at N-terminal region of pHIS2 vector. I have done double restriction digestion of plasmid and observed my target gene on agarose gel electrophoresis. Then I did the...

03 April 2013 9,408 7 View

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