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Questions related from Gnanasekaran Gopalsamy
I am preparing presentation for my upcoming conference in India. Anyone help me to provide information about difficulties, challenges in using miRNA probe for cancer diagnosis. Thanks in advance
02 February 2017 5,643 1 View
I am doing sub-culture of my bacteria (WT) every 16 h in 5 ml LB broth with antibiotic for 30 days. For whole genome sequencing, I should change the LB broth sub-culture 5 ml to 100 ml to collect...
10 October 2015 5,560 2 View
What is the evidence for the generally taught "primordial germinal layer" hypothesis? Is it sufficient to sustain the hypothesis/dogma? Are there any indications that contradict the...
08 August 2014 3,612 8 View
Is this really what happens? If so, the three lineages would give rise to layers in organs such as the colon with three distinct patterns of ancestral somatic mutations. Alternately, organs could...
08 August 2014 257 3 View
I have isolated and identified more than 40 hypothetical proteins from E. coli by using MALDI-TOF and LC-MS/MS. Hypothetical proteins are cloned, over expressed and two proteins are characterized...
04 April 2013 3,005 5 View
I have cloned my gene at N-terminal region of pHIS2 vector. I have done double restriction digestion of plasmid and observed my target gene on agarose gel electrophoresis. Then I did the...
04 April 2013 9,384 7 View