I have tried several solvent systems for TLC of bryophytes on Silica plates. But i haven't got a good separation. Do you have any experience with TLC of bryophyte extracts? Do I have to use reverse plates?
you know that bryophytes refer of group of plants which have different species .what kind of species do you deal with and what are the type of the molecules you want to extract from it?
I have extracts from mosses, liverworts and hornworts. and they were extracted using methanola dn dichloromethane. so primary and secondary metabolites must be there.
To attain a proper solvent system for TLC plate development you may first apply a diluted spot of extract of plant/herb (any solvent you are working) at the centre of a filter paper and with a thin capillary start applying solvents in a sequence (from non polar pet.ether to polar ethanol) .......you can obtain clear circular bands on paper which will give an idea of solvent mixture for TLC separation.
Dear Sameera,
The first step should be to know your species and then look for literature on reported chemical constitutents from them.
Based on the species (or related species) and the solvent of extraction, you could have an idea of what group of chemistries you are dealing with in the extract.
That should provide you the clue to look for separation conditions and detection methods.
There is no need to reinvent the wheel! Once you roughly know the group of chemistries, refer to Wagner and Bladt book on 'Plant Drug Analysis, A Thin Layer Chromatograph' which provides comprehensive information on the TLC systems to follow for specified chemistry groups. You can fine tune the separation conditions for your sample matrix.
Below I have provided a couple of references on bryophyte phytochemistry that could be of help
Yoshinori Asakawa, 2001. Recent advances in phytochemistry of bryophytes-acetogenins, terpenoids and bis(bibenzyl)s from selected Japanese, Taiwanese, New Zealand, Argentinean and European liverworts, Phytochemistry
Volume 56, Issue 3, February Pages 297–312.
Phytochemicals in Human Health Protection, Nutrition, and Plant Defense
Recent Advances in Phytochemistry Volume 33, 1999, pp 319-342
Phytochemistry of Bryophytes
Yoshinori Asakawa
Chemical Constituents of Bryophytes: Bio- and Chemical Diversity, Biological Activity, and Chemosystematics (Progress in the Chemistry of Organic Natural Products) Hardcover – November 13, 2012
by Yoshinori Asakawa (Author), Agnieszka Ludwiczuk (Author), FUMIHIRO NAGASHIMA (Author), Douglas A Kinghorn (Series Editor), Heinz Falk (Series Editor), Junichi Kobayashi (Series Editor)
Good luck
You have used MeOH for extraction - I hope it is aq. MeOH of about 80% - and you are correct that there should be some SMs, however did you go for a biochemical screening for the class of sec. metabolites that might be present before a TLC as you need to use a specific spray reagent. Also try increasing the concentration of you extract.
You may consult Biochemical methods by Harborne
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