Hello, we are designing a plasmid for a vaccine, we want to add an intron in the eukaryotic region to improve transgenic expression. But we do not have a sequence of the intron, I have searched in different articles, but without success. How can I search the sequence? Do you know of any that you could share? Thank you
What species you trying to express in and do you have a specific name for your intron/plasmid? Addgene sometimes has plasmid sequences with introns annotated.
Many plasmids use a b-globin chimeric intron. As Robert Adolf Brinzer mentioned you can find some on addgene for example
https://www.addgene.org/198132/
Dulce María Galván .... Dulce, I don't know. But to gather more insight I suggest using Google Scholar and using "sequence of the intron in the eukaryotic regions" as the search parameters. Google Scholar is found here - https://scholar.google.com/
Thank you for your responses and for your recommendation.
Addgene, it helped me a lot.
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