DOes someone know what is the best basis set and method for calculating excited state of ion CH5+ ??

I've been trying to do my calculations related to optimization of excited state of ion CH5+ using method TD-DFT with M06-2X and aug-cc--pVTZ. My input file is as shown...

03 January 2021 2,148 0 View

Can I ignore the NMAR assumption when missing data is very negligible?

Hello! I am performing preliminary analyses on my data set (N= 187 heterosexual couples). When examining the summary of missing values, SPSS indicates that only 0.16% of my data is missing....

01 December 2020 5,562 13 View

Likert scale over repeated measures?

I am working on research where I have 66 participants who perform an exercise every day, and then they need to respond to 14 questions with a Likert scale (1-10). They performed this exercise for...

20 November 2020 4,682 6 View

Which anesthesia system should I buy to perform stereotaxic surgeries in mice?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...

06 November 2020 3,613 3 View

Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

Ion torrent - What happens if the amplicons are too long?

Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...

11 August 2020 368 3 View

Can we unbias data from umbrella simulations with missing data points?

Imagine a system with 2 energetic basins A and B separated by a high barrier. We cannot sample that barrier for whatever reason (ie. because the lowest energy path is very "long" with lots of...

11 September 2019 9,156 1 View

Emission filter for FLIM to filter off GFP signal?

Dear all, I am measuring changes in fluorescence lifetime of my chromophore in cells expressing also GFP and I would need to filter off the GFP signal. After playing with the parameters in...

11 August 2019 5,297 3 View

Testing for correlation/regression between two scale scores on SPSS?

I am trying to find the correlation/regression between these two variables: Social media usage (measured by 10 items) (total scale score 50) Materialism (measured by 9 items) (total scale score...

10 April 2019 4,123 4 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

01 February 2019 9,590 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View