Hello,

I was hoping someone could point me in the right direction here. I would like to assess the stability of a peptide water-in-oil emulsification since the formulation is performed under high pressure. Initially, I thought that using a recombinant GFP would be a decent readout for peptide stability, since the fluorescence will diminish if the active site is denatured. However, getting recombinant GFP is a bit of a pain and is expensive in the quantities that I need. I was wondering if anyone can recommend something else that can be used as a visual readout for peptide stability? Would a kit like the one sold here be suitable? (https://www.enzolifesciences.com/ENZ-51027/proteostat-thermal-shift-stability-assay-kit/). the only issue is that it seems to mention that it can measure peptide stability as a response to changes in pH, ionic strength, and concentration. In my experiments, the primary source of peptide destabilisation is the high shear pressure that the samples will undergo during the emulsion process. I thought about using other conjugated dyes or fluorophores, but I wasn't sure if they would be good approximations for peptide stability because they may still fluoresce even if the peptide were actually denatured or fragmented. I appreciate any advice here. Thank you kind people!