For MCAO model of stroke
Hello. Good Night Recently in class, students were asked a question that is important to me, and I heard some interesting answers. My question is, how can a woman perform many duties and...
05 December 2022 6,891 8 View
Sohrab Sepehri one of Persian poets says at the end of one of his tender and mystical poems called "water`s footsteps" : It is not our business to fathom the mystery of rose🌹 Perhaps our...
26 November 2022 6,979 90 View
Free Elsevier dental journal
04 May 2019 1,381 1 View
In normal nanofabrication exp., we used to apply PMMA as triple as the final thickness of the required nano-structures in EBL process. My question is , what will happen if we use a higher...
06 July 2016 8,718 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...
30 July 2024 2,349 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View