Can you inject transposase enzyme, along with tol2 plasmid into zebrafish embryos?

07 July 2018 0 5K Report

I have a tol2 flanked construct that I want to inject into zebrafish in the hopes of making a transgenic line. I know that the standard protocol is to inject it with transposase mRNA? I was wondering if I could, instead, use commercially available transposase enzyme?

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