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I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will...
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FBS is harvested from the blood of cattle fetuses as a byproduct of the beef industry. Circulating nucleic acids are DNA and RNA species of unknown oirign which are found in interstitial fluids,...
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I am attempting to analyze nuclear DNA damage induced by an ectopically-expressed endonuclease. This nuclease recognizes an eight-base sequence, and forms a double-stranded break with a two-base...
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In fluorescence microscopy, is it considered best practise to capture grayscale images and artificially colorize, or to capture color images directly?
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