What selection markers to use for the generation of stable HEK293 cell line ?

Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...

14 August 2020 7,542 2 View

What are your approaches and practices regarding games in the EFL classroom?

Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...

28 July 2020 3,077 2 View

How can I export data (csv) from scopus effecient to an excel table?

Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...

13 January 2020 5,916 3 View

How does Burying beetle Nicrophorus vespilloides burying and copulating act look like?

I have read several papers about Nicrophorus vespilloides burying and copulation and I still have some questions. -Do only males bury the carrion? -Does male figth with intruders or also female?...

11 February 2018 8,481 9 View

Do I need permission to collect invertebrates from Gran Canaria?

I am going to Gran Canaria and planning to collect some invertebrates. Do I need permission for that and what should I do for getting the permission? I will collect for natural history museum of...

14 January 2017 5,777 15 View

Would you please identify those freshwater aquarium species?

20 October 2016 5,370 7 View

Can you identify those fish?

Please help me identify those fish. My oppinion 1-3 Taurulus bubalis 4-5 Cyclopterus lumpus

16 June 2016 725 6 View

What species of Mollusca are these specimens?

That species of Mollusca was found from lake Viljandi (https://www.google.ee/maps/@58.3483153,25.6106634,14z?hl=en). This summer. The measurements of shell are shell height 4,5mm shell width...

09 December 2014 2,540 12 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View