Can you help with the estimation of terpenoids and glycosides?

01 January 2014 1 8K Report

Do you have a protocol for total estimation of terpenoids and glycosides using UV spec methodology? I only have a protocol for their estimation using TLC.

Ravi Kant Upadhyay

Terpenes and terpenoids are primary constituents of essential oils of different type of plants and flowers. 1) T Salkowski Test is applied for estimation of terpinoids. For which plant extract is mixed with 2ml of chloroform and concentrate H2SO4 (3ml) is carefully added to form a layer. A reddish brown coloration of the interface is formed to show positive result of the presence of terprnoids.

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Science method

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