After concentration the crude extract (47.5 g) was partitioned between isooctane and MeOH. The MeOH phase was washed with isooctane (8 times) and concentrated. The residue was partitioned between n-BuOH and water. The n-BuOH phase (8.7 g) was washed with water and a portion (3.6 g) was fractionated by gel chromatography.
The above paragraph is the methodology to partition and washing for the extracts before doing column chromatography. I want to know how to do partition and washing of the extracts. Do I need to use any specific glassware or whatmann filter paper or centrifugation? Please can you provide the detailed explanation?
For small amount (microliters) you can use centrifugation, for milliliters people normally use separatory funnels. For instructions you can refer youtube https://www.youtube.com/watch?v=Qlf0G9OGB-I.
For the amount of extract that you have mentioned, you need to use Separatory funnels and the extraction have to be done several times to achieve maximum efficiency.
For good results the crude extract must be completely solved in one of the phase. In the case you described: methanol. Use separatory funnels and work properly in a fume hood. It is necessary to vigorously shake the funnels with both extraction solvents. You must be trained first with technical assistance. When using water, after partition add some dry material to the organic phase (in the case you described: butanol), as anhydride Na2SO4 and filtrate before remove the solvent.
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