I need to design a construct that puts a reporter gene under the regulation of a particular enhancer, in order to quantify the expression (by qPCR) mediated by this enhancer. The problem I have is that I need the expression of a secondary reporter gene under the control of a promoter that's not affected by the enhancer, to normalize the expression.
Is this a viable strategy? If that's the case, I was thinking maybe a CMV promoter? What would you recommend?