Hi to all,
I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file columns in the following format:
column1 column2 column3
nucleotide position wt nucleotide mutated nucleotide
3 A G
Can anyone provide me a python script that replace the nucleotide 3 (A) with the mutated one (G)? Since my wt sequence is in lowercase, mutated nucleotides should be in uppercase to easily identiy them.
Thanks a lot to everyone
Dario