Hi to all,

I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file columns in the following format:

column1 column2 column3

nucleotide position wt nucleotide mutated nucleotide

3 A G

Can anyone provide me a python script that replace the nucleotide 3 (A) with the mutated one (G)? Since my wt sequence is in lowercase, mutated nucleotides should be in uppercase to easily identiy them.

Thanks a lot to everyone

Dario

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