I have a problem with gateway technology and LR reaction.

I Perform LR reaction with various vector concentrations but the reaction is not carryout. I  did check LR enzyme and this enzyme worked good (with control PENTR-lacZ in kit).

Various vector concentrations:

1. pad/v5/dest vector: 150 ng, pentr11/gene containing: 150 ng: total volume: 10µl

2. pad/v5/dest vector: 150 ng, pentr11/gene containing: 100 ng: total volume: 10µl

3. pad/v5/dest vector: 150 ng, pentr11/gene containing: 75 ng: total volume: 10µl

4. pad/v5/dest vector: 50ng, pentr11/gene containing: 25 ng: total volume: 5µl

PAD5/V5/DEST vector length: 36686 bp

PENTR11/GENE containing: 6.7 kb

What is your recommendation?

Please help me

Thanks