How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Has any research on translation investigated the phenomenon of bilingual authors who produce versions of their books in both their languages?

Can the second-language version, strictly speaking, be considered a translation of the first-language version or is it merely a somewhat looser interpretation? How do such authors approach the...

10 December 2020 5,092 25 View

How to distinguish CA1 pyramidal cell vs. interneuron using voltage-clamp?

I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...

29 October 2020 1,098 2 View

Increase Oleophilicity while decreasing hydrophilicity on silicon surfaces?

Hello RG community, currently I am trying to reach these goals on silicon surfaces for a microfludic project. I have used silanes like APTES in the past in order to get my si-surface hydrophobic...

22 March 2020 2,925 3 View

How could one improve the magnitude of t-statistics of a construct?

Hello All, I am not even sure if this is possible, but I have used this dataset for several publication -albeit with different constructs-and have not seen such low t-values. For example, I have a...

10 March 2020 1,164 3 View

How do I calculate the Mean and Standard Deviation for a set of items (i.e., that form a construct) in SPSS?

Hello All, I have been asked to supply the Mean and Standard Deviation for my latent constructs. Is there a straightforward way of doing it in SPSS? I am actually modeling in SmartPLS, but not...

04 March 2020 1,597 4 View

Any strategies for quantification and purification of a red pigment with no peaks in the visible region which is only soluble in water?

We have isolated a red pigment producing bacteria. We harvested the crude pigment by removing the cells by centrifugation. We tried to find the absorption maxima (lambda max) of the pigment using...

19 January 2020 1,331 2 View

How to Create Small Graphs for Interaction Effects?

Hello All, I have been able to calculate all the interaction effects for a study involving the Big Five. One of the interaction effects I would like to graph (among others) is the relationship...

30 December 2019 6,640 3 View

XML haar cascade for red blood cells and/or echinocytes ?

Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...

23 October 2018 3,262 2 View

ITO - PDMS plasma bonding, any idea?

24 May 2018 857 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View